The plaque lipid lysophosphatidic acid stimulates platelet activation and platelet-monocyte aggregate formation in whole blood: involvement of P2Y1 and P2Y12 receptors.

HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY The plaque lipid lysophosphatidic acid stimulates platelet activation and platelet-monocyte aggregate formation in whole blood: involvement of P2Y1 and P2Y12 receptors

Despite the fact that lysophosphatidic acid (LPA) has been identified as a main platelet-activating lipid of mildly oxidized low-density lipoprotein (LDL) and human atherosclerotic lesions, it remains unknown whether it is capable of activating platelets in blood. We found that LPA at concentrations slightly above plasma levels induces platelet shape change, aggregation, and platelet-monocyte a...

Platelet activation and platelet- monocyte aggregate formation induced by the atherosclerotic plaque lipid lysophosphatidic acid

Role of P2Y receptor subtypes in platelet-derived microparticle generation.

Microparticles are shed from the platelet membrane upon platelet activation by strong agonists, and they aid in clot formation. As the P2Y1 and the P2Y12 receptors differentially contribute to different platelet functions, we studied the relative contribution of the P2Y1 and P2Y12 receptors to microparticle formation from platelets. The P2Y12 receptor antagonist AR-C 69931MX, but not the P2Y1 r...

Platelets mediate oxidized low-density lipoprotein-induced monocyte extravasation and foam cell formation.

OBJECTIVE A growing body of evidence indicates that platelets contribute to the onset and progression of atherosclerosis by modulating immune responses. We aimed to elucidate the effects of oxidized low-density lipoprotein (OxLDL) on platelet-monocyte interactions and the consequences of these interactions on platelet phagocytosis, chemokine release, monocyte extravasation, and foam cell format...

HEMOSTASIS, THROMBOSIS, AND VASCULAR BIOLOGY Blockade of adenosine diphosphate receptors P2Y12 and P2Y1 is required to inhibit platelet aggregation in whole blood under flow

Using heparinized whole blood and flow conditions, it was shown that adenosine 5*-diphosphate (ADP) receptors P2Y12 and P2Y1 are both important in direct shearinduced platelet aggregation and platelet aggregation subsequent to initial adhesion onto von Willebrand factor (vWf)– collagen. In the viscometer, whole blood was subjected to shear rates of 750, 1500, and 3000 s21 for 30 seconds at room...